天天做天天干,国产极品粉嫩小泬免费看,国产,各种自啪精品视频,五月婷婷丁香狠

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>AN3 CA人子宮內(nèi)膜腺癌細(xì)胞

人子宮內(nèi)膜腺癌細(xì)胞

簡要描述:HTB-111 AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:AN3 CA
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-09-11
  • 訪  問  量:2390

產(chǎn)品分類

Product Category

詳細(xì)介紹

HTB-111 AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞

ATCC® Number: HTB-111™ Price: $399.00

Designations: AN3 CA

Depositors: CJ Dawe

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Proper sapiens (human)

Morphology: epties: adherent

Organism: Homoithelial

HTB-111 AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞

Source: Organ: uterus

Tissue: endometrium

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12,14,15

D13S317: 12,14

D16S539: 10,14

D5S818: 11,14

D7S820: 7,10,7.1

THO1: 10,9.3

TPOX: 8,10

vWA: 14,20

Isoenzymes: AK-1, 1-2

ES-D, 1

G6PD, B

GLO-I, 2

PGM1, 1

PGM3, 1-2

Age: 55 years

Gender: female

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Volumes used in this protocol are for 75 sq. cm flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22517: Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105



HTB-111 AN3 CA





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
信宜市| 休宁县| 策勒县| 德阳市| 肇源县| 宁陵县| 临漳县| 筠连县| 桦南县| 前郭尔| 苏尼特右旗| 霍邱县| 广州市| 汉阴县| 曲松县| 星座| 拜城县| 安义县| 平阴县| 大悟县| 汉川市| 松滋市| 玉山县| 河间市| 宜君县| 资源县| 宝清县| 白沙| 新乡市| 二连浩特市| 铁岭县| 琼中| 上杭县| 吐鲁番市| 黎川县| 武邑县| 清丰县| 花莲县| 徐汇区| 黄浦区| 磐石市|